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The PGI1 gene (blue) in the yeast genome is targeted for disruption by the <t>natMX6</t> cassette (orange), which confers resistance to NAT. Homologous recombination at the flanking regions (indicated by Xs) facilitates the replacement of the PGI1 gene with the natMX6 selectable marker.
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The PGI1 gene (blue) in the yeast genome is targeted for disruption by the <t>natMX6</t> cassette (orange), which confers resistance to NAT. Homologous recombination at the flanking regions (indicated by Xs) facilitates the replacement of the PGI1 gene with the natMX6 selectable marker.
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The PGI1 gene (blue) in the yeast genome is targeted for disruption by the <t>natMX6</t> cassette (orange), which confers resistance to NAT. Homologous recombination at the flanking regions (indicated by Xs) facilitates the replacement of the PGI1 gene with the natMX6 selectable marker.
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The PGI1 gene (blue) in the yeast genome is targeted for disruption by the <t>natMX6</t> cassette (orange), which confers resistance to NAT. Homologous recombination at the flanking regions (indicated by Xs) facilitates the replacement of the PGI1 gene with the natMX6 selectable marker.
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Plasmids used in this study.
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The PGI1 gene (blue) in the yeast genome is targeted for disruption by the natMX6 cassette (orange), which confers resistance to NAT. Homologous recombination at the flanking regions (indicated by Xs) facilitates the replacement of the PGI1 gene with the natMX6 selectable marker.

Journal: bioRxiv

Article Title: dnaudit + pydnaweb: A lightweight text-based planning and documentation workflow for genetic cloning with automatic verification

doi: 10.1101/2025.05.31.657172

Figure Lengend Snippet: The PGI1 gene (blue) in the yeast genome is targeted for disruption by the natMX6 cassette (orange), which confers resistance to NAT. Homologous recombination at the flanking regions (indicated by Xs) facilitates the replacement of the PGI1 gene with the natMX6 selectable marker.

Article Snippet: Briefly, the natMX6 cassette from the pAG25 plasmid (Addgene plasmid # 35121; 20) was amplified by PCR using primer 383_up-PGI1 and primer 382_dn-PGI1 ( Table S1 ).

Techniques: Disruption, Homologous Recombination, Marker

Plasmids used in this study.

Journal: Frontiers in Microbiology

Article Title: The gene YEF3 function encoding translation elongation factor eEF3 is partially conserved across fungi

doi: 10.3389/fmicb.2024.1438900

Figure Lengend Snippet: Plasmids used in this study.

Article Snippet: pAG25-NATMX , Contains the NATMX gene DNA that confers resistance to the antibiotic cloNAT. , Addgene, plasmid ID #35121.

Techniques: Plasmid Preparation, Cloning, PCR Cloning, Marker, Expressing, Clone Assay, Sequencing